Fig. 6: mRNA-seq profiling in NCOA2-knockdown BMDMs treated with Ad-HNF4A, IL-4 and Dex. | Cell Death & Disease

Fig. 6: mRNA-seq profiling in NCOA2-knockdown BMDMs treated with Ad-HNF4A, IL-4 and Dex.

From: HNF4A mitigates sepsis-associated lung injury by upregulating NCOA2/GR/STAB1 axis and promoting macrophage polarization towards M2 phenotype

Fig. 6

A BMDMs infected with Ad-HNF4A are infected with Ad-shNCOA2 or Ad-shNC for 48 h, and then cells are cultured with IL-4 and Dex for 24 h followed by transcriptome analysis. B Principal-component analysis (PCA) analysis. C Volcano plot of differentially expresses genes (DEGs). D The heatmap of DEGs. E GO and KEGG enrichment analysis of DEGs. F Venn diagram shows that overlapping peak is identified between anti-GR and anti-NCOA2 binding peaks in ChIP-seq-GSE99887 in the left panel. The middle Venn diagram shows that overlapping genes between overlapping genes of left Venn diagram and DEGs in mRNA-seq. The right Venn diagram shows that overlapping genes between the top 10% of Genecards-retrieval involved in macrophage polarization and overlapping genes of middle Venn diagram. The data are presented as mean ± SD.

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