Fig. 4: TRAP1 point mutations differentially affect SDH activity and mitochondrial bioenergetics. | Cell Death & Disease

Fig. 4: TRAP1 point mutations differentially affect SDH activity and mitochondrial bioenergetics.

From: Point mutations of the mitochondrial chaperone TRAP1 affect its functions and pro-neoplastic activity

Fig. 4

a Succinate dehydrogenase (SDH) activity measured in sMPNST cells expressing either human wild-type or mutant forms of TRAP1. Data are reported as mean±SEM of 3 independent experiments with a two-tail unpaired Student’s t-test with each mutant compared to hTRAP1-WT expressing cells (**p value < 0,01; ***p value < 0,001; n.s. non-significant). b TRAP1 immunoprecipitation in murine sMPNST cells re-expressing either the wild-type or the mutant forms of human TRAP1 after knocking-out endogenous TRAP1. c Root mean square fluctuation (RMSF) per residue considering the backbone atoms only. The mutated positions are indicated with an arrow. On the x axis numbering of zTrap1 is as in PDB code 4IPE and is shown together with the corresponding domains of protomer A and protomer B. For each protomer, residue numbering is from 85 to 719. d–f Quantification of basal oxygen consumption rate (OCR; d), mitochondrial ATP production (e) and extracellular acidification (ECAR; f) measured in sMPNST cells expressing either human wild-type or the mutant forms of TRAP1. Data are reported as mean±SD of 3 independent experiments, with each mutant compared to hTRAP1-WT expressing cells; asterisks indicate significant differences (∗∗p < 0.01, ∗p < 0.05; Student’s t-test analysis).

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