Fig. 3: Downregulation of LCN2 alleviated LPS-related neuronal damage.
From: LCN2 induces neuronal loss and facilitates sepsis-associated cognitive impairments
A Representative immunofluorescence images show astrocyte activation and LCN2 fluorescence in primary hippocampal astrocytes. B Quantitative analysis of LCN2 fluorescence in primary hippocampal astrocytes (n = 9–10). C LCN2 protein levels were detected in primary hippocampal astrocytes by immunoblotting, actin was used as a loading control, and D quantitative analysis of the LCN2, (n = 4). E ELISA kit was used to measure the LCN2 levels in ACM, (n = 4). F Primary hippocampal neurons were treated with Control-ACM, LPS-ACM, AAV-Sh-LCN2-ACM, and the MAP2 was measured by immunofluorescence. Sholl analysis (Scale bar: 20 μm), (n = 10) (G) and quantitative analyses of dendritic length (n = 15) (H) were performed. Data are presented as Mean ± SEM. one-way ANOVA was used for statistical analysis. *p < 0.05, **p < 0.01, ****p < 0.0001, versus LPS group.
