Fig. 5: Large tumors with HIS-low harbored fewer immunosuppressive cells compared with large tumors with HIS-high. | Cell Death & Disease

Fig. 5: Large tumors with HIS-low harbored fewer immunosuppressive cells compared with large tumors with HIS-high.

From: High baseline levels of PD-L1 reduce the heterogeneity of immune checkpoint signature and sensitize anti-PD1 therapy in lung and colorectal cancers

Fig. 5

A MCA38-pCDH and MCA38-PD-L1 tumors were collected when they reached 4.0–5.0 mm and 7.0–8.0 mm in diameter, respectively. Intratumoral immune cells were analyzed by flow cytometry. B The representative flow cytometry plots of CD4+, CD8+ T cells, and CD4+CD25+ Tregs. The proportions of intratumoral CD4+ T cells (C), CD8+ T cells (D), Tregs (E), CD4+PD1+ T cells (F), CD8+PD1+ T cells (G), M-MDSCs (CD45+CD11b+Gr1medF4/80med, H), PMN-MDSCs (CD45+CD11b+Gr1hiF4/80−, I), and TAMs (CD45+CD11b+Gr1−F4/80+, J) were shown. Data were from one experiment representative of two independent experiments with similar results. Each dot represents one individual tumor sample. Data are shown as the mean ± SEM and were analyzed by two-way ANOVA.

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