Fig. 1: K⁺ currents and membrane potentials in 3T3-L1 cell line during adipogenesis.

A Schematic experimental design. 3T3-L1 preadipocyte were differentiated into mature adipocyte for 7 days. Imaging ORO staining, western blotting, and patch clamp recording were conducted. B representative images of 3T3-L1 cell morphology (left). The cells were used for measuring K⁺ channel conductance using voltage-clamp recording at ND, D3, D5, and D7 (right). Command voltage pulse protocol: voltages were stepped from −100 mV to +100 mV in increments of 10 mV from a holding potential of −40 mV. The dotted line indicates 0 pA. C The current–voltage relationship (I–V) from −100 mV to +100 mV. Representative whole-cell passive conductance of adipocytes in preadipocytes and mature adipocytes through the 3T3-L1 cell line. D Bar graph showing the amplitude of each condition from −100 mV to +100 mV through preadipocytes and mature adipocytes. Data are mean ± s.e.m. One-way ANOVA to obtain p-values. ***P < 0.001. The number of slices tested in at least 12 independent cells is indicated in each bar. E Resting membrane potential (RMP) on 3T3-L1 comparison before and after differentiation. Data are mean ± s.e.m. P-values were obtained with Student’s t test. ***P < 0.001.