Fig. 2: Potassium current and membrane potentials with TREK-1 antagonist treatment in 3T3-L1 cell line while adipocytes are differentiating.
From: Novel function of TREK-1 in regulating adipocyte differentiation and lipid accumulation
A The mRNA expression of KCNK families in ND and D5 during adipocyte differentiation. B Expression of KCNK2 mRNA by time of differentiation. C Representative traces of K+ current recordings for Spadin (1 μM) treatment in 3T3-L1 preadipocytes (left), Spadin treatment (right). D Representative traces of differentiated adipocyte K+ current recordings. Differentiation (left) and Spadin treatment (right). E, F Current densities induced by voltage ramping were recorded in differentiated adipocytes (blue) and Spadin-treated (purple). Spadin-sensitive current (red). G RMP on 3T3-L1 comparison before and after Spadin (1 μM) treatment. H Oil Red O staining image to measure cellular lipid droplets. Scale bars indicate 20 μm and 10 μm, respectively. I Quantification of ORO staining in 3T3-L1 cells treated with Spadin from D3 to D7. Data are mean ± s.e.m. P-values were obtained with one-way ANOVA ****P < 0.0001.
