Fig. 2: Ferroptosis-related damage occurs in RPE cells in laser-induced CNV in mice.
From: The role of vitamin K and its antagonist in the process of ferroptosis-damaged RPE-mediated CNV
A Protein levels of RPE65 (a marker of RPE) and vWF (a marker of endothelial cells) in mice-RPE and HUVEC were examined by western blot. B–D Level of Fe2+, ratio of GSH/GSSG, and level of MDA in RPE in mice treated as indicated were measured by detection kits. E, F Protein level of 4-HNE in RPE in mice treated as indicated was determined by immunofluorescence. G, H Protein levels of FTH1, TFR1, and ACSL4 in RPE in mice treated as indicated were examined by western blot. The data represent the averages of three independent experiments. Data shown are mean ± SD; one-way ANOVA with Bonferroni correction; **P < 0.01. Scale bars: 10 μm.
