Fig. 5: ERRα regulates glucose and cholesterol metabolism to suppress the autophagy–lysosomal pathway. | Cell Death & Disease

Fig. 5: ERRα regulates glucose and cholesterol metabolism to suppress the autophagy–lysosomal pathway.

From: High glucose levels promote glycolysis and cholesterol synthesis via ERRα and suppress the autophagy–lysosomal pathway in endometrial cancer

Fig. 5: ERRα regulates glucose and cholesterol metabolism to suppress the autophagy–lysosomal pathway.

A In the HEC-1A and KLE cells, the expression of HK2, p62 treated with 2-DG after ERRα overexpressing in the HG environment was shown by immunofluorescence (Magnification: 80×). B In the HEC-1A and KLE cells, the expression HMGCS1, p62 treated with simvastatin was presented by immunofluorescence staining after ERRα up-regulating in the HG environment (Magnification: 80×). C Relative fluorescence intensity of ERRα, HK2, and p62 when treated with 10 mM 2-DG for 24 h under high glucose in HEC-1A and KLE cells. D Relative fluorescence intensity of ERRα, HMGCS1, and p62 when treated with 5 μM and 10 μM simvastatin for 24 h under high glucose in HEC-1A and KLE cells, respectively. E, F TEM images of ASSs treated with 2-DG or simvastatin for 24 h under high glucose in KLE and HEC-1A cells, respectively (Magnification: 8000×). G, H The ASSs counts of HEC-1A and KLE cells treated with 2-DG or simvastatin for 24 h under high glucose after ERRα overexpressed, respectively. n = three independent experiments, *P < 0.05, **P < 0.01 or ***P < 0.001. EC endometrial cancer, Mnt mannitol, NG normal glucose, HG high glucose, TEM transmission electron microscopy, 2-DG 2-deoxy-d-arabino-hexose.

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