Fig. 3: Analysis of potential HRH1-regulated pathways involved in head and neck squamous cell carcinoma (HNSCC) progression. | Cell Death & Disease

Fig. 3: Analysis of potential HRH1-regulated pathways involved in head and neck squamous cell carcinoma (HNSCC) progression.

From: Cyclic increase in the histamine receptor H1-ADAM9-Snail/Slug axis as a potential therapeutic target for EMT-mediated progression of oral squamous cell carcinoma

Fig. 3

A Heatmap displaying expressions of differentially expressed genes among the top 5% of TCGA-HNSCC patients with the highest or lowest HRH1 expression. B Horizontal bar plot illustrating the top 10 enriched gene sets in the HRH1-high group, utilizing the gene set database, h.all.v7.5.symbols.gmt [Hallmark]. C Representative enrichment plots for transforming growth factor (TGF)-β signaling and the EMT, which are significantly positively associated with high HRH1 expression. The EMT gene set was derived from h.all.v7.5.symbols.gmt [Hallmark]. D Volcano plot illustrates genes that are downregulated (FC of <0.7, FDR of <0.05; 1194 genes represented by blue dots) and upregulated (FC of >1.5, FDR of <0.05; 567 genes represented by red dots) in SAS/shHRH1 cells. E GSEA of altered genes in shHRH1 versus shCtrl groups. F Ingenuity pathway analysis (IPA) of shHRH1 versus shCtrl in SAS cells for the top 10 significant upstream regulators of SAS/shHRH1 cells. G Heatmap indicates highly positive associations between HRH1 expression and TGF-β1-regulated genes in SAS cells. H Correlation plot demonstrates correlations between HRH1 gene expression and TGF-β as well as EMT markers. RNA sequencing data from TCGA-HNSCC patients were utilized for this analysis, with correlation coefficients and p values evaluated through a Pearson correlation analysis. TCGA the cancer genome atlas, EMT epithelial-to-mesenchymal transition, GSEA gene set enrichment analysis, FC fold change, FDR false discovery rate.

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