Fig. 3: Successful breeding of Aeg-1fl/flCre+ mice. | Cell Death & Disease

Fig. 3: Successful breeding of Aeg-1fl/flCre+ mice.

From: Hippocampus- and neocortex-specific deletion of Aeg-1 causes learning memory impairment and depression in mice

Fig. 3: Successful breeding of Aeg-1fl/flCre+ mice.

A Breeding strategy of Aeg-1fl/flCre+ mice. B–D PCR identification of sites in Aeg-1fl/flCre+ mice: (B) PCR identification of 5′ screening site (Fl/fl = 570 bp, Fl/wt = 570 bp/471 bp, Wt = 471 bp); (C) PCR identification of D5-5 site (Fl = 527 bp, Wt = none); (D) PCR identification results of Cre site (T = 481 bp); 1-20: Aeg-1fl/wtCre+ mice number; wt: WT mice; N: blank control; Marker bands: 700 bp/600 bp/500 bp/400 bp/300 bp/200 bp/100 bp. E, F Western blot results of AEG-1 protein expression in Aeg-1fl/flCre+ mice and WT mice: E Western blotting analysis of AEG-1 and the quantification was presented in (F). HP brain hippocampal tissue, CTX brain cortical tissue, SPL kidney tissue. Results analyzed by Tukey’s test. *p < 0.05, ***p < 0.001, ns: no significant. G Immunofluorescence detection of AEG-1 protein expression in the hippocampus and cortex of Aeg-1fl/flCre+ mice and WT mice. Scale Bar: 200 μm.

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