Fig. 1: Podocyte senescence induced by adriamycin.
From: NAT10 mediates TLR2 to promote podocyte senescence in adriamycin-induced nephropathy
A Enrichment plots of P53 signaling pathway in podocytes before and after adriamycin stimulation. The stimulation condition of adriamycin was 0.5 μg/mL/12 h. B WB was used to detect P53, P21, P16, and γH2AX(S139) expression in the CON group and ADR15 group in kidney tissue (n = 6). C WB was used to detect P53, P21, P16, and γH2AX(S139) expression in the CON group and ADR group in podocytes (n = 3). D Densitometric analysis of (B). The relative intensities of the bands were normalized to the intensities of the respective β-actin signal. E Densitometric analysis of (C). F Representative micrographs showing SA-β-gal staining in podocytes. The scale bars = 20 μm. G The quantification of the SA-β-gal-positive cells was determined after three biological replicates. H WB was used to detect NAT10 expression in the CON group and ADR15 group in kidney tissue (n = 6). I Densitometric analysis of NAT10. J Immunohistochemistry was utilized to detect the expression of NAT10. The scale bars = 20 μm.
