Fig. 2: Remodelin alleviates adriamycin-induced Podocytopathy.
From: NAT10 mediates TLR2 to promote podocyte senescence in adriamycin-induced nephropathy
A Representative micrographs show the expression of podocin in different groups with glomeruli. The experiment was performed using frozen slices with a thickness of 5 μm. The scale bars = 20 μm. The electron microscope tissue was obtained from the apical cortex of the mouse kidney. The magnification of the electron microscope is 50,000 times and the scale bars = 200nm. B WB was used to detect NAT10, WT-1, and Nephrin expression in the CON group, ADR group, and ADR+RH group in kidney tissue (n = 6). C Densitometric analysis of NAT10. D Densitometric analysis of WT-1. E Densitometric analysis of Nephrin. F WB was used to detect NAT10 and Synaptopodin expression in the CON group, ADR group, and ADR+RH group in podocytes (n = 3). G Densitometric analysis of NAT10. H Densitometric analysis of Synaptopodin. I WB was used to detect NAT10 expression in different siRNA treatment conditions. J WB was used to detect NAT10 and Synaptopodin expression in different groups (n = 3). K Densitometric analysis of NAT10. L Densitometric analysis of Synaptopodin. M, N Immunofluorescence staining of F-ACTIN, red represents F-actin and blue represents DAPI, the scale bars = 50 μm.
