Fig. 5: Inhibition of SET8 reduces DDR in the kidney and TKPTs following cisplatin (CP) treatment.
From: SET8 inhibition preserves PTEN to attenuate kidney cell apoptosis in cisplatin nephrotoxicity
Mice were treated with cisplatin and UNC0379 (UNC) as indicated in Fig. 1A. Kidney tissue lysates were prepared and subjected to immunoblot analysis using antibodies as indicated (A). The levels of all the proteins were quantified by densitometry, and γ-H2A, p-p53, and p21 were normalized with H2A, p53, and Tubulin, respectively, (B). TKPTs were treated with UNC0379 for 1 h (C–F) or transfected with siRNA targeting SET8 (siSET8) or control siRNA (siCON) (G–J) for 24 h and then exposed to cisplatin for 24 h. Cell lysates were prepared and subjected to immunoblot analysis using antibodies as indicated (C, G). The levels of all the proteins were quantified by densitometry. γ-H2A (D), p-p53 (E), and p21 (F) were normalized with H2A, p53, and GAPDH, respectively, and γ-H2A (H), p-p53 (I), and p21(J) were normalized with H2A, p53, and Tubulin, respectively. Data are represented as the mean ± SEM of at least three experiments. *P < 0.05, **P < 0.01, ***P < 0.001.
