Fig. 5: Characterization of extracellular vesicles secreted by cell lines.

a Phenotypic characterization of extracellular vesicles isolated from supernatant of exhausted culture media of cell lines obtained by FACS analysis. Histograms represent the mean fluorescence intensity (MFI) of the detected membrane proteins with values > 500 in at least one of the samples. MFI values are reported in the side table. b Cluster analysis and heatmap representation of CTC and commercial GEC cell lines based on the 14 miRNAs contained into EV and differentially expressed between the two groups.