Fig. 3: GFAP-positive neurons emerge in the mouse frontal association cortex after spinal cord transection. | Cell Death & Disease

Fig. 3: GFAP-positive neurons emerge in the mouse frontal association cortex after spinal cord transection.

From: SGK1 upregulation in GFAP+ neurons in the frontal association cortex protects against neuronal apoptosis after spinal cord injury

Fig. 3

A, B Representative horizontal section images of the hippocampus (A) and frontal association cortex (FrA, B) of an SCT-treated KI mouse and its sham surgery control after tissue clearing without immunostaining (PEGASOS). Scale bar, 500 μm. C Representative horizontal section images of the whole brain of an SCT-treated KI mouse and its sham surgery control after immunostaining-compatible tissue clearing (iDISCO) with antibodies against GFAP (green) and NeuN (red). GFAP+ NeuN- cells were indicated with magenta arrows whereas GFAP+ NeuN+ cells were indicated with white arrows. Scale bar, 500 μm. D Statistical analysis of GFAP+ NeuN+ cell ratios in all GFAP+ cells within the FrA sections of sham/SCT-treated KI mice. ***p < 0.001 vs. GFAP KI-Sham-FrA; n = 5 distinct transverse sections.

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