Fig. 5: tRF-22 regulates VM formation in GBM by negatively regulating the expression of MXD1. | Cell Death & Disease

Fig. 5: tRF-22 regulates VM formation in GBM by negatively regulating the expression of MXD1.

From: TRMT10A regulates tRNA-ArgCCT m1G9 modification to generate tRNA-derived fragments influencing vasculogenic mimicry formation in glioblastoma

Fig. 5: tRF-22 regulates VM formation in GBM by negatively regulating the expression of MXD1.The alternative text for this image may have been generated using AI.

Changes in MXD1 expression were verified by RT-qPCR (A) and western blot (B), respectively, after silencing or overexpressing MXD1 in U-251 MG and T98G cells. Values are presented as mean ± SD (n = 3), **P < 0.01, ***P < 0.001  compared to the NC group. C Cell proliferation was assessed using the CCK-8 assay after 48 h of culture (n = 5). D Cell migration and invasion abilities were evaluated using the Transwell assay after 48 h of culture (n = 3). E Tube formation ability of cells was assessed using the tube formation assay (n = 3). Values are presented as mean ± SD, **P < 0.01 compared to the sh-NC group; ##P < 0.01 compared to the OE-NC group. Scale bar = 50 μm. F After 48 h of transfection with tRF-22 inhibitor in MXD1 stable knockdown U-251 MG and T98G cells, cell proliferation was assessed using the CCK-8 assay (n = 5). G Cell migration and invasion abilities were evaluated using the Transwell assay (n = 3). Scale bar = 50 μm. H Tube formation ability of cells was assessed using the tube formation assay (n = 3). Values are presented as mean ± SD, **P < 0.01 compared to the inhibitor NC group; ##P < 0.01 compared to the tRF-22 inhibitor + sh-NC group. Scale bar = 50 μm.

Back to article page