Fig. 5: TRIM21 promotes ferroptosis by targeting METTL3 in vitro. | Cell Death & Disease

Fig. 5: TRIM21 promotes ferroptosis by targeting METTL3 in vitro.

From: TRIM21-mediated METTL3 degradation promotes PDAC ferroptosis and enhances the efficacy of Anti-PD-1 immunotherapy

Fig. 5

A Flow cytometry analysis was used to determine cell death rates in subgroups of METTL3-knockdown cells treated with various cell death inhibitors. B The electron microscopy images show changes in mitochondria following treatment with shMETTL3 in MIA PaCa-2 cells. Scale bar, 2.0μm and 500 nm. C RIP-qPCR revealed the binding enrichment of SLC7A11 to METTL3 in MIA PaCa-2 cells. Western blot was used to verify the immunoprecipitation efficiency of METTL3 protein. D, E MeRIP-qPCR analysis demonstrated the impact of METTL3 on mediating m6A methylation modification of SLC7A11 mRNA in pancreatic cancer cells. F, G The SLC7A11 mRNA expression was measured by RT-qPCR in pancreatic cancer cells with the intervention of TRIM21 and METTL3. H–K The Altered protein expression of SLC7A11 was measured by western blot in subgroups. L The cell death induced by TRIM21 overexpression was effectively rescued by METTL3 overexpression or treatment with ferroptosis inhibitors ferrostatin-1 (1 μM). (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, ns not significant).

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