Table 2 Primer sequences for Trpm8 chromatin.
From: Dietary targeting of TRPM8 rewires macrophage immunometabolism reducing colitis severity
Primer | Sequence |
|---|---|
methylated forward 1 (MF1) | ATGTTTAGAGGATTAGTGAATACGT |
methylated reverse 1 (MR1) | AACTAAAAACACCATAAAAATCGTA |
unmethylated forward 1 (UF1) | ATGTTTAGAGGATTAGTGAATATGT |
unmethylated reverse 1 (UR1) | AAACTAAAAACACCATAAAAATCATA |
methylated forward 2 (MF2) | ATGTTTAGAGGATTAGTGAATACGT |
methylated reverse 2 (MR2) | AACTAAAAACACCATAAAAATCGTA |
unmethylated forward 2 (UF2) | ATGTTTAGAGGATTAGTGAATATGT |
unmethylated reverse 2 (UR2) | AACTAAAAACACCATAAAAATCATA |
methylated forward 3 (MF3) | ATGTTTAGAGGATTAGTGAATACGT |
methylated reverse 3 (MR3) | AACTAAAAACACCATAAAAATCGTA |
unmethylated forward 3 (UF3) | ATGTTTAGAGGATTAGTGAATATGT |
unmethylated reverse 3 (UR3) | ACTAAAAACACCATAAAAATCATA |