Fig. 5: BCAT2 inhibits autophagy-related ferroptosis in PCa cells. | Cell Death & Disease

Fig. 5: BCAT2 inhibits autophagy-related ferroptosis in PCa cells.

From: BCAT2 binding to PCBP1 regulates the PI3K/AKT signaling pathway to inhibit autophagy-related apoptosis and ferroptosis in prostate cancer

Fig. 5

A CCK-8 assay for shNC or shBCAT2-2 cells, treated with or without 2 μM Fer-1 (MedChemExpress, US). B WB analysis of ferroptosis related protein levels in PCa cells. C, D ROS staining of PCa cells. E The lipid ROS level (C11-BODIPY® 581/591) of PCa cells was analyzed by a flow cytometer. F Mitochondrial membrane potential (TMRE staining) of PCa cells. G, H The levels of MDA and GSH/GSSG in PCa cells pretreated. I, J The levels of intracellular iron measured in PCa cells pretreated with or without 5 mM 3-MA for 24 h. K WB analysis of ferroptosis related protein levels in shNC or shBCAT2-2 cells, treated with or without 5 mM 3-MA for 24 h.

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