Fig. 2: GLI2 depresses CDDP sensitivity and promotes GC cell metastasis via EMT in vitro. | Cell Death & Disease

Fig. 2: GLI2 depresses CDDP sensitivity and promotes GC cell metastasis via EMT in vitro.

From: GLI2 inhibits cisplatin sensitivity in gastric cancer through DEC1/ZEB1 mediated EMT

Fig. 2: GLI2 depresses CDDP sensitivity and promotes GC cell metastasis via EMT in vitro.

A Western blot analysis of GLI2 expression in GC cell lines and normal gastric epithelial cells. B, C MKN-45 cells with stable GLI2 overexpression or HGC-27 cells with GLI2 knockdown were created. The changes in GLI2 expression were confirmed using qPCR or western blot. D–F Western blot analysis investigated the effect of GLI2 on the expression of indicated proteins combined with CDDP treatment. G CCK-8 experiment shows the IC50 values for CDDP in indicated cells. H Transwell experiment tested the impact of GLI2 on the migratory and invasion ability of GC cells under CDDP treatment. Scale bars: 50 µm. ****P < 0.0001, ***P < 0.001, **P < 0.01, *P < 0.05.

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