Fig. 8: GLI2 and DEC1 form a protein complex that can cooperatively activate ZEB1 expression.
From: GLI2 inhibits cisplatin sensitivity in gastric cancer through DEC1/ZEB1 mediated EMT
A, B The possible GLI2 and DEC1 binding sites in human ZEB1 promoter. Transcriptional activity of ZEB1 was measured by the luciferase reporter system. Reporter activity is reported as the fold activation relative to Renilla luciferase activity. C ZEB1 promoter binding sites with GLI2 and DEC1 were validated by ChIP. D Co-IP of GLI2 and DEC1. Flag-tagged GLI2 or DEC1 transcripts were transfected into MKN-45 cells. *P < 0.05.
