Fig. 2: GL-V9 alleviates lung inflammation and inhibits M2 macrophage polarization in mice with bleomycin-induced pulmonary fibrosis. | Cell Death & Disease

Fig. 2: GL-V9 alleviates lung inflammation and inhibits M2 macrophage polarization in mice with bleomycin-induced pulmonary fibrosis.

From: Inhibition of Rho GEFs attenuates pulmonary fibrosis through suppressing myofibroblast activation and reprogramming profibrotic macrophages

Fig. 2: GL-V9 alleviates lung inflammation and inhibits M2 macrophage polarization in mice with bleomycin-induced pulmonary fibrosis.

Mice with pulmonary fibrosis induced by transoral instillation of Bleomycin and treated with GL-V9 at 300 mg/kg on an every-other-day dosing regimen. Lung specimens and BALF were harvested on days 7, 14, and 21 after the establishment of the model. A Total number of cells in BALF from mice. B Macrophage polarization status in BALF from mice on day 7. Flow cytometry analysis of the percentage of infiltrated M2 macrophages (F4/80+ CD206+) and M1 macrophages (F4/80+ CD86+) were shown. Differences between groups were analyzed using unpaired t-test. C The relative mRNA levels of MRC1 and ARG1 in the right lung. D The concentration of TGF-β1 in BALF. E Immunohistochemical staining for CD206 in the left lung, with quantification of CD206-positive cell count. Scale bar for the main image: 2.5 mm; enlarged section scale bars: 100 µm. Data are shown as mean ± SEM. ns p > 0.05, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.

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