Fig. 5: RAs induced by miR-423-3p in H-GDEs promoted the progression of glioma in vivo. | Cell Death & Disease

Fig. 5: RAs induced by miR-423-3p in H-GDEs promoted the progression of glioma in vivo.

From: Inhibition of hypoxic exosomal miR-423-3p decreases glioma progression by restricting autophagy in astrocytes

Fig. 5

A, B Bioluminescence imaging of tumor-bearing mice treated with P3#GBM together with PBS-NHA, N-GDEs-NHA, and H-GDEs-NHA. Representative images and statistical analysis of day 28 are shown. C Survival analysis for orthotopic xenografts bearing P3#GBM together with PBS-NHA, N-GDEs-NHA, and H-GDEs-NHA (five mice per group). D H&E staining (scale bar, 300 μm) and IHC staining (scale bar, 100 μm) for Ki67 and GFAP from xenograft mice treated with P3#GBM, PBS-NHA, N-GDEs-NHA, and H-GDEs-NHA on the day of euthanasia. Representative images of three sets of experiments are shown. E Co-immunofluorescence staining for LC3B (green) and GFAP (red) in NHAs in orthotopic xenografts treated with PBS, N-GDEs, and H-GDEs derived from P3#GBM after euthanasia. Cell nuclei were stained with DAPI (blue). Representative images of three sets of experiments are shown (scale bar, 10 μm). F, G Bioluminescence imaging of tumor-bearing mice treated with P3#GBM together with lenti-control-NHA and lenti-ov-miR-423-3p-NHA. Representative images and statistical analysis of day 28. H Survival analysis for orthotopic xenografts bearing P3#GBM together with lenti-control-NHA and lenti-ov-miR-423-3p-NHA (five mice per group). I, J H&E staining (scale bar, 300 μm) and IHC staining (scale bar, 100 μm) for Ki67 and GFAP from xenograft mice co-planted with lenti-control-NHA and lenti-ov-miR-423-3p-NHA. Representative images of three sets of experiments and statistical analysis for IHC score of Ki67 are shown. K Co-immunofluorescence staining for LC3B (green) and GFAP (red) in lenti-control-NHAs and lenti-ov-miR-423-3p-NHAs. Cell nuclei were stained with DAPI (blue). Representative images of three sets of experiments are shown (scale bar, 10 μm). (Data reflects the mean ± SEM. *P < 0.05; **P < 0.01; ***P < 0.001; n = 3). GBM glioblastoma multiforme, H-GDE hypoxic glioma-derived exosome, N-GDE normoxic glioma-derived exosome, NHA normal human astrocyte, RA reactive astrocytes.

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