Fig. 2: Soluble oligomeric Aβ42 causes reduced proteasome activity in neurons. | Cell Death & Disease

Fig. 2: Soluble oligomeric Aβ42 causes reduced proteasome activity in neurons.

From: Modulation of CREB3L2-ATF4 heterodimerization via proteasome inhibition and HRI activation in Alzheimer’s disease pathology

Fig. 2

A Validation of the proteasome activity assay. Proteasome activity assays on lysates of cortical neurons treated with vehicle or Aβ42 for 8 hours. Peptidase activity of the 20S proteasome was measured at 120 minutes using specific fluorogenic substrates (50 µM): Suc-LLVY-AMC (chymotrypsin-like; B), Z-LLE-AMC (caspase-like; C), or Z-ARR-AMC (trypsin-like; D). Means ± SEM of n = 7 (B) or 9 (C, D) independent biological replicates. Unpaired, two-tailed t-tests. *p < 0.05; **p < 0.01.

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