Fig. 2: Silencing ROBO1 expression improved ESCC cells radio-sensitivity. | Cell Death & Disease

Fig. 2: Silencing ROBO1 expression improved ESCC cells radio-sensitivity.

From: ROBO1 enhanced esophageal carcinoma cell radioresistance through accelerating G3BP2-mediated eIF3A degradation

Fig. 2

A The influence of radiation on ROBO1 expression detected by Western blot. B, C The knockdown efficiency of ROBO1 in ESCC cells was determined by a western blot and RT-qPCR assays. D, E ESCC cell proliferation status was evaluated by clone formation assays and CCK-8 assays after radiation exposure. F Representative immunofluorescence images of γ-H2A.X distribution in cells with or without ROBO1 depletion under radiation condition. G The influence of ROBO1 knockdown on γ-H2A.X expression upon radiation treatment. H Impact of ROBO1 silencing on cell apoptosis detected by flow cytometry analysis. I The expression of apoptosis-associated protein was detected in ROBO1-knockdown cells by Western blot.

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