Fig. 6: ROBO1 stimulated mTOR signaling pathway through blocking eIF3A-mediated P53 translation. | Cell Death & Disease

Fig. 6: ROBO1 stimulated mTOR signaling pathway through blocking eIF3A-mediated P53 translation.

From: ROBO1 enhanced esophageal carcinoma cell radioresistance through accelerating G3BP2-mediated eIF3A degradation

Fig. 6

A Pathway enrichment analysis for the influence of ROBO1 knockdown in ESCC. B, C The influence of ROBO1 silence on P53 expression was detected at protein level and mRNA level. **p < 0.01. D P53 protein degradation and translation was blocked in ESCC cells with or without ROBO1 depletion. MG132, a proteasome inhibtor. CHX, a translation blockade. E ROBO1 regulated the translation of P53 with the help of eIF3A. F The influence of EIF3A on regulating P53 translation and stability was explored by Western blot. G Silencing P53 initiated mTOR signaling cascades and induced downstream DNA nucleotide excision repair (NER) genes expressions in response to irradiation treatment. H–J The influence of G3BP2, ROBO1 or eIF3A abrogation on mTOR signaling activity was explored in ESCC cells upon irradiation exposure.

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