Fig. 5: MMP1 promotes EMT and proliferation by activating NF-κB pathway in BC cells. | Cell Death & Disease

Fig. 5: MMP1 promotes EMT and proliferation by activating NF-κB pathway in BC cells.

From: MMP1-induced NF-κB activation promotes epithelial–mesenchymal transition and sacituzumab govitecan resistance in hormone receptor-positive breast cancer

Fig. 5: MMP1 promotes EMT and proliferation by activating NF-κB pathway in BC cells.

a Effects of MMP1 overexpression and knockdown in parental and resistant cell lines on the NF-κB pathway and EMT markers. MMP1, TNF, pIKKα/β, IKKβ, pP65, P65, pIκB, IκB, AKT, p-AKT, and EMT-related protein markers were detected by Western blot. b MCF-7-Pa and T47D-Pa cells were transfected with a vector or MMP1, followed by treatment with the NF-κB inhibitor PDTC for 24 h. The expression levels of TNF, MMP1, pIKKα/β, IKKβ, pP65, P65, pIκB, IκB, AKT, p-AKT, and EMT-related protein markers were assessed via Western blot. GAPDH served as an internal control. c, d Transwell assays were conducted respectively in MCF-7-Pa and T-47D-Pa cells transduced with a vector or MMP1 to evaluate the effects of MMP1 or the NF-κB inhibitor PDTC on cell migration and invasion. e, f Wound scratch assays were performed respectively in MCF-7-Pa and T-47D-Pa cells transduced with a vector or MMP1 to assess the effects of MMP1 or the NF-κB inhibitor PDTC on cell migration. g, h Colony formation assays were utilized respectively to detect differences in proliferation ability under MMP1 overexpression with or without PDTC treatment in MCF-7-Pa and T-47D-Pa. UT, untreated. * p < 0.05, ** p < 0.01, *** p < 0.001.

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