Fig. 1: Gsdmd is expressed in macrophages, glomerular cells, and presumably in injured proximal tubular cells that fail to undergo repair in the kidney, and gene sets related to macrophage migration are upregulated in a chronic oxalate nephropathy mice model. | Cell Death & Disease

Fig. 1: Gsdmd is expressed in macrophages, glomerular cells, and presumably in injured proximal tubular cells that fail to undergo repair in the kidney, and gene sets related to macrophage migration are upregulated in a chronic oxalate nephropathy mice model.

From: Gasdermin D deficiency aggravates nephrocalcinosis-related chronic kidney disease with rendering macrophages vulnerable to necroptosis

Fig. 1

A Dot plot representing the expression of Gsdmd in each kind of cells in the kidney. The dot color indicates the average gene expression level in each cluster, while the dot size represents the percentage of cells in each cluster. The bar plots on the top of the box are the numbers of cells in each group. B Pseudotime trajectory of proximal tubular cells in uIRI mouse model, colored by cluster identity. C Expression levels of Gsdmd in each cluster of proximal tubular cells seven days after uIRI in trajectory analysis. D Supervised pseudotime analysis of Gsdmd gene expression levels in each cluster of proximal tubular cells after uIRI. The x-axis represents pseudotime, and the y-axis shows z-scored gene expression values. E Representative images of Gsdmd staining in the kidneys of WT mice fed a control diet, and WT and Gsdmd−/− mice fed an oxalate-rich diet. F The enrichment plots from the GSEA analysis show gene enrichment profiles comparing WT mice fed an oxalate-rich diet versus a control diet. The plots highlight the enrichment of transcriptional signatures related to macrophage migration. G Density ridge plots showing the distribution of gene expression for core-enriched genes in enriched gene sets, with gradient colors indicating adjusted p-values using the Benjamini-Hochberg method. CNT: connecting tubule, CTAL2: thick ascending limb of loop of Henle in cortex, DCT: distal convoluted tubule, ATL: thin ascending limb of loop of Henle, EC: endothelial cells, Fib: fibroblasts, ICA: type A intercalated cells of collecting duct, MTAL: thick ascending limb of loop of Henle in medulla, Mφ: macrophages, PT: proximal tubule, PC: principle cells, PTS1: S1 segment of proximal tubule, PTS2: S2 segment of proximal tubule, PTS3: S3 segment of proximal tubule, Pod: Podocytes, Uro: Urothelium.

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