Fig. 2: Gasdermin D knockout (Gsdmd−/−) mice subjected to an oxalate-rich diet manifest a worsened renal outcome in comparison to WT mice. | Cell Death & Disease

Fig. 2: Gasdermin D knockout (Gsdmd−/−) mice subjected to an oxalate-rich diet manifest a worsened renal outcome in comparison to WT mice.

From: Gasdermin D deficiency aggravates nephrocalcinosis-related chronic kidney disease with rendering macrophages vulnerable to necroptosis

Fig. 2

A Study design illustration. Eight-week-old WT (n = 15) and Gsdmd−/− mice (n = 13) received an oxalate rich diet for 21 days. The mice were sacrificed on day 21 for subsequent analyses. B Glomerular filtration rate (GFR) at various time points in WT and Gsdmd−/− mice. C Blood Urea Nitrogen (BUN) levels on day 21 in WT mice fed a control diet and in WT and Gsdmd−/− mice fed an oxalate-rich diet. D mRNA expression of KIM-1, TGFβ1, and TNFα in kidney RNA isolates. Data are presented as mean ± SD. P values were calculated using two-way ANOVA with Bonferroni’s multiple comparisons test (B), one-way ANOVA with Turkey’s post-hoc test (C), or Mann-Whitney U test (D). n.s., not significant; *P < 0.05, **P < 0.01, ***P < 0.001.

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