Fig. 5: Immunobot analysis and serum IL-1β level suggest limited involvement of pyroptosis, while histological analysis shows no contribution of apoptosis to kidney injury in CaOx crystal-induced chronic nephropathy. | Cell Death & Disease

Fig. 5: Immunobot analysis and serum IL-1β level suggest limited involvement of pyroptosis, while histological analysis shows no contribution of apoptosis to kidney injury in CaOx crystal-induced chronic nephropathy.

From: Gasdermin D deficiency aggravates nephrocalcinosis-related chronic kidney disease with rendering macrophages vulnerable to necroptosis

Fig. 5

A Representative TUNEL staining images from kidneys of WT mice fed a control diet, and of WT and Gsdmd−/− mice fed an oxalate-rich diet. B Quantification of TUNEL positive cells. C Representative cleaved caspase-3 staining images from kidneys of WT mice fed a control diet, and of WT and Gsdmd−/− mice fed an oxalate-rich diet. D Quantification of cleaved caspase-3 positive cells. E Immunoblot analysis of Gsdmd, caspase-1 (pro and cleaved p20), IL-1β (pro and mature p17), Gsdme (pro and cleaved p35) in the kidneys of WT and Gsdmd−/− mice treated with an oxalate-rich diet. β-actin was used as a loading control. F IL-1β levels in serum from WT and Gsdmd−/− mice fed an oxalate-rich diet. uIRI: unilateral ischemia-reperfusion injury. Data are presented as mean ± SD. P values were calculated using one-way ANOVA with Turkey’s post-hoc test (B, D, F). n.s., not significant; *P < 0.05.

Back to article page