Fig. 8: Gsdmd−/− macrophages are vulnerable to CaOx crystal-induced damage, leading to an elevated occurrence of necroptosis. | Cell Death & Disease

Fig. 8: Gsdmd−/− macrophages are vulnerable to CaOx crystal-induced damage, leading to an elevated occurrence of necroptosis.

From: Gasdermin D deficiency aggravates nephrocalcinosis-related chronic kidney disease with rendering macrophages vulnerable to necroptosis

Fig. 8

A Representative images of BMDMs stained with SYTOX Green (SG) 4 h after stimulation with CaOx crystals. B Quantification of SG-positive cells at 4 h after stimulation with CaOx crystals. C BMDMs were stimulated with 1 000 μg/ml CaOx crystals for 18 h, and cell-free supernatants were collected for LDH assay. D Representative immunofluorescent images of BMDMs 18 h after stimulation with 1 000 μg/ml CaOx crystals. Green: pMLKL, Red: F4/80, Blue: 4’,6-diamidino-2-2phenylindole (DAPI). E Quantification of pMLKL positive areas in BMDMs treated with CaOx for 18 h. F After pretreatment with or without Nec-1s or GSK872 for 1 h, BMDMs were stimulated with 1 000 μg/ml CaOx crystals for 18 h. Cell-free supernatants were collected for LDH assay. Data are presented as mean ± SE from at least 3 independent experiments. P values are calculated using one-way ANOVA with Turkey’s post-hoc test (B, E) or Mann-Whitney U test (C) or two-way ANOVA with Bonferroni’s multiple comparisons test (F). *P < 0.05.

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