Fig. 2: ICD and cGAS-STING pathway activation in the treated Hepa1-6 cells. | Cell Death & Disease

Fig. 2: ICD and cGAS-STING pathway activation in the treated Hepa1-6 cells.

From: Oncolytic peptide LTX-315 plus an anti-CTLA-4 antibody induces a synergistic anti-cancer immune response in residual tumors after radiofrequency ablation of hepatocellular carcinoma

Fig. 2

A, B Western blotting was used to assess the expression of ICD proteins (ATP, CALR, HMGB1). C, D Western blotting was applied to measure the expression of cGAS-STING pathway proteins (cGAS, STING, p-TBK1, and p-IRF3). E–H qRT-PCR was used to measure the expression of TNF-α, CXCL9, CXCL10, and IFN-β. I–L ELISA was applied to evaluate the levels of TNF-α, CXCL9, CXCL10, and IFN-β in the cell culture medium. n = 3 in each group. Error bars represent standard deviation. ***p < 0.001. ICD immunogenic cell death, iRFA incomplete radiofrequency ablation, CTLA-4 cytotoxic T lymphocyte antigen-4, qRT-PCR quantitative real-time polymerase chain reaction, ELISA enzyme-linked immunosorbent assay.

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