Fig. 2: IEC-specific Rap2B deletion inhibits colorectal cancer tumorigenesis. | Cell Death & Disease

Fig. 2: IEC-specific Rap2B deletion inhibits colorectal cancer tumorigenesis.

From: Rap2B drives tumorigenesis and progression of colorectal cancer through intestinal cytoskeleton remodeling

Fig. 2: IEC-specific Rap2B deletion inhibits colorectal cancer tumorigenesis.The alternative text for this image may have been generated using AI.

A Schematic representation of strategies used to generate Rap2Bflox/flox and Rap2BIEC-KO mice. B Genotyping PCR analysis using tail DNAs from Rap2BIEC-WT, Rap2BIEC-+/FL and Rap2BIEC-KO showing the flox and WT alleles of Rap2B and the Cre transgene. C, D Representative immunoblot analysis and IHC staining confirming loss of Rap2B expression in the colon tissue of Rap2BIEC-KO mice. E Schematic of the experimental strategy to induce CRC model. F Representative images of colons from Rap2BIEC-WT and Rap2BIEC-KO mice with CRC induction model. Zoomed images of boxed region are shown at the left. G, H Quantification of tumor incidence per colon and colon length of Rap2BIEC-WT and Rap2BIEC-KO mice with CRC induction model (n = 13). I Representative H&E analysis of the colons from Rap2BIEC-WT and Rap2BIEC-KO mice with CRC induction model. J–L Representative micrographs of immunochemistry staining for Ki-67 and Claudin-3 in tumors from Rap2BIEC-WT and Rap2BIEC-KO mice with CRC induction model (n = 5). Data in J–H, K and L are presented as the mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001 by 2-tailed unpaired t test.

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