Fig. 9: NDUFS8 depletion sensitizes ionizing radiation-induced cytotoxicity in primary NSCLC cells. | Cell Death & Disease

Fig. 9: NDUFS8 depletion sensitizes ionizing radiation-induced cytotoxicity in primary NSCLC cells.

From: NADH:ubiquinone oxidoreductase core subunit S8 expression and functional significance in non-small cell lung cancer

Fig. 9

The primary human NSCLC cells, pNSCLC1, stably expressing lentivirus-packed shNDUFS8-S1, the scramble control shRNA (“shC”) (A–C), lentivirus-packed Cas9 construct plus the CRISPR/Cas9-NDUFS8-KO construct (“koNDUFS8”) or the control construct (“koC”) (D–F), lentivirus-packed NDUFS8-overexpressing construct (“oeNDUFS8”) or the empty vector (“Vec”) (G–I) were treated with or without ionizing radiation (IR, 4 Gy) for indicated time periods, cell viability, death and apoptosis were tested via CCK8 (A, D, G), Trypan blue staining (B, E, H) and TUNEL nuclear staining (C, F, I) assays, respectively, with results quantified. “Ctrl” stands for the untreated control. Error bars in the figures represent the mean ± standard deviation (SD), with statistical significance (*P < 0.05) when comparing “Ctrl” cells. #P < 0.05. The experiments depicted in this figure were independently repeated five times (n = 5) and consistently yielded similar results.

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