Fig. 6: AZIN1-mediated polyamine production alters osteosarcoma immunogenicity and enhances T-cell-mediated cytotoxicity. | Cell Death & Disease

Fig. 6: AZIN1-mediated polyamine production alters osteosarcoma immunogenicity and enhances T-cell-mediated cytotoxicity.

From: AZIN1-dependent polyamine synthesis accelerates tumor cell cycle progression and impairs effector T-cell function in osteosarcoma

Fig. 6: AZIN1-mediated polyamine production alters osteosarcoma immunogenicity and enhances T-cell-mediated cytotoxicity.The alternative text for this image may have been generated using AI.

A Gene set enrichment analysis (GSEA) of RNA-seq data from 143B cells, showing changes in immune response-related gene expression with and without AZIN1 knockdown. B Heatmap of transcriptional levels of genes regulating T cell responses in 143B cells, under conditions with and without AZIN1 knockdown. C ELISA quantification of IFN-γ levels in T cell co-cultures with 143B-A2M4 cells, comparing AZIN1 knockdown with control conditions. Data are expressed as mean ± SD (n = 3). Statistical evaluation was performed using one-way ANOVA. D Representative image of HLA-A/B/C staining in 143B-A2M4 orthotopic tumors, gated on live and HLA-A/B/C positive cells, with mean fluorescence intensity (MFI) quantification analysis. Data are presented as mean ± SD (n = 4). Statistical significance was determined using an unpaired t-test. E Quantitative PCR (qPCR) analysis of T cell activation-related gene expression in 143B cells with and without AZIN1 knockdown. Data are expressed as mean ± SD, n = 3. Statistical analysis was performed using two-way ANOVA. F qPCR analysis of HLA-A and IL1A expression levels in 143B cells post-AZIN1 knockdown and supplemented with either putrescine (put) or spermidine (spd). Data are expressed as mean ± SD, n = 3. Statistical analysis was conducted using one-way ANOVA. G Assessment of TCR-T cell-mediated cytotoxicity against 143B-A2M4 cells with AZIN1 knockdown and supplementation with 10 μM putrescine (put) or 5 μM spermidine (spd). Data are presented as mean ± SD, n = 3. Statistical evaluation utilized one-way ANOVA. H Normalized expression levels of NFKBIA in RNA-seq data from 143B and U2-OS cells under conditions with and without AZIN1 knockdown. Data are presented as mean ± SD (n = 3). Statistical significance was assessed using an unpaired t-test. Significance levels are denoted as follows: ***P ≤ 0.001, **P ≤ 0.01, *P ≤ 0.05, n.s. not significant.

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