Fig. 6: TTC36S125D but not TTC36S125A exerts its oncogenic role in HCC.
A, B LO2 or PLC/PRF/5 cells with Flag-TTC36 were subjected to Colony Formation assay, sorafenib concentration gradient was 0, 5, 10, 15, 20, 25, and the same volume of DMSO was used as a blank control. C, D In vitro growth of LO2 or PLC/PRF/5 cells treated with R of Flag-TTC36 by Cell Counting Kit-8. E Analysis of TTC36 phosphorylation levels in the TCGA-LIHC cohort. F The target plasmid was transfected into LO2, PLC/PRF/5, and Huh7 cells as shown in the figure, and then Western blotting was performed using the corresponding antibodies in the figure. G HEK-293T cells were transfected with plasmids as shown in the figure, treated with MG-132 (10 μM) for 6 h before cell collection with Guanidine hydrochloride lysis buffer, immunoprecipitated with Ni-NTA affinity resin, and immunoblotted with specified antibodies. H, J Colony formation assay of PLC/PRF/5 or Huh7 under the conditions illustrated. I, K Evaluation of in vitro growth of PLC/PRF/5 or Huh7 cells under the conditions depicted in the figure using the Cell Counting Kit-8.
