Fig. 7: The oncogenic effect of TTC36 is dependent on c-Myc in HCC.
A, B The effects of si-TTC36 inhibition or knockout on the in vitro growth of hepatocellular carcinoma cells were evaluated. Potential blocking of this inhibition by Flag-c-Myc was evaluated using Cell Counting Kit-8. C, D The effects of TTC36 overexpression on the in vitro growth of Huh7 or LO2 cells were evaluated. Potential blocking of this inhibition by si-c-Myc was evaluated using Cell Counting Kit-8. E, F A colony formation assay was used to detect whether Flag-c-Myc would hinder the growth-promoting effect of TTC36 inhibition or knockout-induced hepatoma cells. G, H A colony formation assay was used to detect whether si-c-Myc would hinder the growth-promoting effect of TTC36 overexpression-induced hepatoma cells. I Hydrodynamic injection of HCC original cancer mouse model. J Gross liver anatomy and tumor formation in wild-type and TTC36 knockout mice (4 weeks after injection). Liver weight (K), Liver/Body weight ratio (L), Tumor number(M), Tumor weight (N) of adult WT, and TTC36−/− mice (n = 7; mean ± SD). O Representative images of H&E, TTC36, Ki67, and c-Myc immunostaining of wild-type and TTC36 knockout mouse liver sections. P Immunoblotting of liver lysates from wild-type (WT) and TTC36−/− C57BL/6 mice using the antibodies shown in the figure; GADPH served as the loading control (N = 7). Q Work model.
