Fig. 1: U-251 MG S, U-251 MG R and T98-G cells differently tolerate TMZ.
From: Purine metabolism rewiring improves glioblastoma susceptibility to temozolomide treatment
a Schematic representation of the protocol used to induce TMZ resistance in U-251 MG S cells. b–d LDH assay at 24 h on U-251 MG S (b), U-251 MG R (c) and T98-G (d) cells treated with 0, 50, 100, 250, 500 μM of TMZ. e–g Surviving fraction (SF) at 24 h and 72 h post-treatment, quantification of average clones size and representative pictures of the clonogenic assay at 72 h of U-251 MG S (e), U-251 MG R (f) and T98-G (g) clonogenic assay. Data are shown via scattered dot plots as mean ± SD of n ≥ 3 independent experiments. *p-value < 0.05; **p-value < 0.01; **** p-value < 0.0001. CTRL control, FC fold change, SF surviving fraction, TMZ temozolomide, Tx-100 Triton X-100.
