Fig. 6: Guanosine and inosine, in combination with TMZ, stimulate mitochondrial fusion.
From: Purine metabolism rewiring improves glioblastoma susceptibility to temozolomide treatment
a Representative micrographs of MitoView staining in CTRL, TMZ-treated and TMZ + GUA + INO-treated cells for U-251 MG S, U-251 MG R, T98-G. Scale bar = 10 μm. b–d Correlated quantification between total branch length/mito and number of individual mitochondrial particles in FC over CTRL for U-251 MG S (b), U-251 MG R (c) and T98- G (d). For U-251 MG S, CTRL: n = 37 cells from 4 biological replicates; TMZ: n = 33 cells from 4 biological replicates; TMZ + GUA + INO: n = 37 cells from 4 biological replicates. For U-251 MG R, CTRL: n = 26 cells from 4 biological replicates; TMZ: n = 45 cells from 4 biological replicates; TMZ + GUA + INO: n = 34 cells from 4 biological replicates. For T98-G, CTRL: n = 39 cells from 4 biological replicates; TMZ: n = 46 cells from 4 biological replicates; TMZ + GUA + INO: n = 40 cells from 4 biological replicates. Data are shown via scatter plots as mean ± SD. CTRL control, FC fold change, GUA guanosine, INO inosine, TMZ temozolomide.
