Fig. 3: TSRP suppresses the PI3K/Akt/mTOR pathway to regulate bone metastasis in the A549 xenograft model with PIK3CA mutations. | Cell Death & Disease

Fig. 3: TSRP suppresses the PI3K/Akt/mTOR pathway to regulate bone metastasis in the A549 xenograft model with PIK3CA mutations.

From: Therapeutic efficacy of TMTP1-modified EVs in overcoming bone metastasis and immune resistance in PIK3CA mutant NSCLC

Fig. 3

A Fluorescence intensity results in NSCLC tumor tissues of mice in each group; B Bioluminescence imaging results of NSCLC tumors and metastatic lesions in vivo in mice of each group (arrows point to areas of bone damage); C Fluorescence intensity results of NSCLC tumor metastasis to hind limb tumor tissues in mice of each group; D H&E staining of tumor tissue and EdU immunofluorescence imaging results of tumor cells, scale bar = 200 μm; E Statistical results of EdU experiment; F, G Volcano plot and heatmap of transcriptome sequencing differential analysis of tumor tissues metastasized to bones in mice from the PBS group (N = 6) and TSRP group (N = 6); H, I Detection results of protein phosphorylation levels of the PI3K/Akt/mTOR pathway in tumor tissues metastasized to bones in mice of each group; J Fluorescence intensity results in NSCLC tumor tissues of mice in each group; K Bioluminescence imaging results of NSCLC tumors and metastatic lesions in vivo in mice of each group (arrows point to areas of bone damage) and fluorescence intensity results of hind limb tumor tissues; L H&E staining of tumor tissue, scale bar = 200 μm and EdU immunofluorescence imaging results of tumor cells, scale bar = 25 μm; M Statistical results of EdU experiment. Each group consisted of 6 mice, * represents p < 0.05.

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