Fig. 2: In vivo administration of MNU inhibits mitophagy in photoreceptors.
From: Deferiprone protects against photoreceptor degeneration by inhibiting parthanatos
a Mitochondria were labeled with anti-TOMM20 (red) in whole-eye retina sections from MNU-treated mice and volume (µm3) of mitochondrial mass was quantified in (b) the retina and (c) the ONL. d Representative images of retina cryosections immunostained with the lysosome marker anti-LAMP1 (cyan) and quantification of lysosome number in (e) ONL and (f) OLM. g Adult (3-6 months old) mito-QC mice were injected intraperitoneally with MNU (60 mg/kg) or vehicle (n = 8–9 per group) and euthanized 24 h later for histological analyses of the retina. Left side of cartoon illustrates the construct carried by mito-QC transgenic mice consisting of a mitochondrial outer membrane-localized tandem mCherry-GFP tag, MTS mitochondria targeting sequence. h Representative images of mito-QC retina cryosections showing mitochondria (yellow) and mitolysosomes (red). Quantification of mitolysosomes in (i) ONL and (j) OLM. Dots represent individual retinas from different mice. All data are expressed as the mean ± SEM. *p < 0.05; **p < 0.01; ***p < 0.001. Statistical analyses were performed using a two-tailed Student´s t-test (for normally distributed data) and non-parametric Mann-Whitney U test (for non-normally distributed data). Scale bars: 25 µm.
