Fig. 8: Nobiletin suppresses cell growth of ESCC in vitro and in vivo. | Cell Death & Disease

Fig. 8: Nobiletin suppresses cell growth of ESCC in vitro and in vivo.

From: ZIP8 modulates ferroptosis to drive esophageal carcinoma progression

Fig. 8: Nobiletin suppresses cell growth of ESCC in vitro and in vivo.The alternative text for this image may have been generated using AI.

A Proliferation of KYSE30, KYSE450, and KYSE510 cells measured by CCK-8 assays at 24 h, 48 h, 72 h, and 96 h intervals. B, C Colony formation assays and crystal violet staining to assess the proliferation abilities of KYSE30, KYSE450, and KYSE510 cells. D, E Anchorage-independent cell growth assay to evaluate the effect of Nobiletin (0 μM, 10 μM, 20 μM, 40 μM) on cell growth (scale bar, 200 μm). F Schematic diagram of the patient-derived xenograft (PDX) model. G, H Tumor size and volume measurements in the PDX model following Nobiletin treatment. I Body weights of mice across different treatment groups. J IHC staining for BAX, Ki67, ZIP8, p-CREB and GPX4 on PDX mouse tissues. IOD SUM represents the integrated optical density value of statistics. Data presented as mean ± S.D. from three independent experiments. Statistical significance was assessed using Student’s unpaired t-test (A, C, E, H, I, J). *p < 0.05, **p < 0.01, **p < 0.001.

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