Fig. 2: TSPYL5 augments the malignant characteristics of NB cells. | Cell Death & Disease

Fig. 2: TSPYL5 augments the malignant characteristics of NB cells.

From: TSPYL5-driven G3BP1 nuclear membrane translocation facilitates p53 cytoplasm sequestration via accelerating RanBP2-mediated p53 sumoylation and nuclear export in neuroblastoma

Fig. 2

A CCK-8 assays showing the sensitivity to cisplatin in the SK-N-SH and SH-SY5Y cells transfected with shNC or shTSPYL5. B Flow cytometry assays showing the apoptosis induced by cisplatin in the SK-N-SH and SH-SY5Y cells transfected with shNC and shTSPYL5. C Colony formation assays in the SK-N-SH and SH-SY5Y cells transfected with shNC or shTSPYL5. D Sphere formation assays in the SK-N-SH and SH-SY5Y cells transfected with shNC or shTSPYL5. Scale bar = 25 μm. E Side population ratio assays in the SK-N-SH and SH-SY5Y cells transfected with shNC and shTSPYL5. F, G Migration and invasion assays in the SK-N-SH and SH-SY5Y cells transfected with shNC and shTSPYL5. Scale bar = 50 μm. Rescue assays was used to exclude the off-target effect. ns = no significant difference. The mean ± SD from three experiments was plotted. *p < 0.05, **p < 0.01, ***p < 0.001.

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