Fig. 7: Exogenous LC suppresses pro-inflammatory polarization of macrophages, while exogenous PC suppresses anti-inflammatory polarization of macrophages. | Cell Death & Disease

Fig. 7: Exogenous LC suppresses pro-inflammatory polarization of macrophages, while exogenous PC suppresses anti-inflammatory polarization of macrophages.

From: Adipocyte-expressed SIRT3 manipulates carnitine pool to orchestrate metabolic reprogramming and polarization of macrophages

Fig. 7

DMDMs were cultured in the presence or absence of LC for 12 h. The mRNA expression of Tnf-α (A) Il-6 (B) Il-1β (C), iNos (D) and Mcp-1 (E) in BMDMs. The secretion of NO (F) TNF-α (G) IL-6 (H) and MCP-1 (I) in the culture supernatant from BMDMs. J The F4/80/CD11c double positive macrophages were analyzed by flow cytometry. ##P < 0.01, ###P < 0.001, LPS vs. control; *P < 0.05, **P < 0.01, ***P < 0.001, LPS vs. LPS + LC. DMDMs were cultured in the presence or absence of PC 12 h. The mRNA expression of Mrc1 (K) Mgl2 (L) Ym1 (M) and Arg1 (N) in BMDM. n = 6 independent experiments. O The F4/80/CD206 double positive macrophages were analyzed by flow cytometry. n = 3 independent experiments. ##P < 0.01, ###P < 0.001, IL-4 vs. control; *P < 0.05, ***P < 0.001, IL-4 vs. IL-4 + PC. $$P < 0.01, $$$P < 0.001, PC vs. control. Data are expressed as means ± SEM.

Back to article page