Fig. 9: SIRT3 decreases the accumulation of PC through deacetylating CPT2. | Cell Death & Disease

Fig. 9: SIRT3 decreases the accumulation of PC through deacetylating CPT2.

From: Adipocyte-expressed SIRT3 manipulates carnitine pool to orchestrate metabolic reprogramming and polarization of macrophages

Fig. 9

A The acetylated CPT2 levels in vector and SIRT3OE adipocytes. n = 3 independent experiments. B The CPT2 activity in vector and SIRT3OE adipocytes. n = 6 replicates. C The CPT2 activity in Scramble and SIRT3KD adipocytes. *P < 0.05, Scramble vs. SIRT3KD. n = 3 replicates. D The substrate dependency in vector and SIRT3OE adipocytes was determined by Seahorse XF mitochondrial fuel flex test. n = 3 replicates. E The CPT2 activity was evaluated. n = 6 replicates. The contents of PC (F) and LC (G) in CM of adipocytes. n = 3 replicates. The mRNA expression of Tnf-α (H), Il-6 (I), Il-1β (J), iNos (K) and Mcp-1 (L) in BMDMs treated with CM of adipocytes. n = 6 replicates. M The protein expression of p-IKKα/β, IKKα, IKKβ, p-IκBα, IκBα, p-p65 and p65. β-actin was used as a loading control. n = 3 replicates. Data are expressed as means ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001, vector vs. SIRT3OE; #P < 0.05, ##P < 0.01, ###P < 0.001, vector vs. CPT2KD; &P < 0.05, & &P < 0.01, control vs vector; $P < 0.05, $$$P < 0.001, CPT2KD vs. SIRT3OE-CPT2KD.

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