Fig. 8: KIFC1 stability is regulated by USP25 and can regulate downstream MYCBP expression.
From: USP25-driven KIFC1 regulates MYCBP expression and promotes the progression of cervical cancer
A–C Co-immunoprecipitation with labeled antibodies showed the interaction between HA-USP25, Flag-KIFC1 and His-MYCBP. D, E USP25-depleted did not affect the mRNA expression difference of KIFC1 and MYCBP in CCa cells. F, G USP25-depleted significantly down-regulated the expression levels of KIFC1 and MYCBP in CCa cells. H, I The expression levels of USP25, KIFC1 and MYCBP proteins in the USP25-depleted CCa cells with different KIFC1 expression levels were analyzed in immunoblotting. J The proposed working model. USP25 deubiquitination maintains the stability of KIFC1 protein, and subsequently KIFC1 can regulate the expression level of downstream MYCBP to play a carcinogenic role. *p < 0.05, **p < 0.001, ***p < 0.001, ****p < 0.0001 by Student’s unpaired two-sided t-test.
