Fig. 3: METTL1 promotes PTC cell proliferation and metastasis in a manner dependent on its tRNA methyltransferase activity. | Cell Death & Disease

Fig. 3: METTL1 promotes PTC cell proliferation and metastasis in a manner dependent on its tRNA methyltransferase activity.

From: METTL1-mediated m7G tRNA modification drives papillary thyroid cancer progression and metastasis by regulating the codon-specific translation of TNF-α

Fig. 3

a Western blot was performed to detect METTL1 protein expression in BCPAP and K1 cells stably transfected with METTL1 wild-type construct (METTL1-WT), catalytic mutation construct (METTL1-MUT), or empty vector (EV). b Cell viability assay by CCK-8 method to compare the proliferation rate between METTL1-WT, METTL1-MUT, and EV stably expressed BCPAP and K1 cells (n = 3). c Representative images and statistical bar graphs of the colony formation assays in METTL1-WT, METTL1-MUT, or EV stably expressed BCPAP and K1 cells (n = 3). d, e Representative images (upper) and statistical bar graphs (lower) depicting the relative cell migration rate (d) and invasion rate (e) in METTL1-WT, METTL1-MUT, or EV stably expressed BCPAP and K1 cells (n = 3). Scale bar = 200 μm f–h Representative images (f), tumor growth volume (g), and tumor weight (h) of subcutaneous xenograft tumors in K1 cells stably transfected with METTL1-WT, METTL1-MU,T or EV. (n = 6). i Representative immunohistochemistry (IHC) staining images and semiquantitative analyses of Ki-67 (upper) and METTL1 (lower) of subcutaneous xenograft tumors in METTL1-WT, METTL1-MUT, or EV stably expressed K1 cells. Scale bar = 50 μm. Two-tailed paired Student’s t-test in (c–e), two-tailed unpaired Student’s t-test in (h, i), One-way ANOVA test in (b, g), ns non-significant, *P < 0.05, **P < 0.01, ***P < 0.001.

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