Fig. 6: TNF-α is essential for METTL1-driven progression and metastasis. | Cell Death & Disease

Fig. 6: TNF-α is essential for METTL1-driven progression and metastasis.

From: METTL1-mediated m7G tRNA modification drives papillary thyroid cancer progression and metastasis by regulating the codon-specific translation of TNF-α

Fig. 6

a METTL1-depleted and control TPC-1 and IHH4 cells were stimulated with 50 ng/ml exogenous TNF-α recombinant protein (r-TNF-α) and vehicle for 48 h, and then compared for cellular viability by the CCK8 method (n = 3). b Representative images and statistical bar graphs of the colony formation assays for METTL1-depleted and control TPC-1 and IHH4 cells with or without stimulation of 50 ng/ml exogenous r-TNF-α for 48 h (n = 3). c, d Representative images (upper) and statistical bar graphs (lower) depicting the relative cell migration rate (c) and invasion rate (d) in METTL1-depleted and control TPC-1 and IHH4 cells with or without stimulation of 50 ng/ml exogenous r-TNF-α for 48 h (n = 3). Scale bar = 200 μm. e Cell viability assay by CCK-8 method to compare the proliferation rate between wild-type TPC-1 and IHH4 cells with or without stimulation of 1.5 μM R-7050 for 6 h (n = 3). f Representative images and statistical bar graphs of the colony formation assays for wild-type TPC-1 and IHH4 cells with or without stimulation of 1.5 μM R-7050 for 6 h. (n = 3). g, h Representative images (left) and statistical bar graphs (right) depicting the relative cell migration rate (g) and invasion rate (h) in wild-type TPC-1 and IHH4 cells with or without stimulation of 1.5 μM R-7050 for 6 h (n = 3). Scale bar = 200 μm. Two-tailed paired Student’s t-test in (b–d and f–h), One-way ANOVA test in (a, e) *P < 0.05, **P < 0.01, ***P < 0.001.

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