Fig. 4: Adipocyte OGT facilitates differentiation of hematopoietic stem cells into mature monocytes. | Cell Death & Disease

Fig. 4: Adipocyte OGT facilitates differentiation of hematopoietic stem cells into mature monocytes.

From: Adipocytes regulate monocyte development through the OGT-NEFA-CD36/FABP4 pathway in high-fat diet-induced obesity

Fig. 4

A ORO staining and quantification of BMAds from siControl and siOGT group. And the statistical histograms were shown on the right. Data reported as from n  = 3 independent biological replicates. B BODIPY 493/503 staining and quantification of BMAds from siControl and siOGT group. And the statistical histograms were shown on the right. Data reported as from n  = 3 independent biological replicates. C Schematic drawing adipocytes and HSCs were co-cultured in transwell. D Schematic drawing hematopoietic stem cell differentiation. E The statistical histogram of LSKs, LT-LSKs, ST-LSKs, and MPPs proportion of BM in mice. Data reported as from n  = 3 independent biological replicates. F The statistical histogram of MP, GMP, MEP, CLP and CMP proportion of BM in mice. Data reported as from n  = 3 independent biological replicates. G The statistical histogram of MONO and NEU proportion of BM in mice. Data reported as from n  = 3 independent biological replicates. H Schematic of the sgRNAs targeting OGT in 3T3L-1 cells. The sgRNAs were designed for KO targeting the third exon of OGT gene. I The statistical histogram of hematopoietic stem cells and their subgroups of cells of BM in mice. Data reported as from n  = 3 independent biological replicates. J The mRNA levels of PU.1 of LSKs in siControl groups and siOGT groups. Data reported as from n  = 3 independent biological replicates. Data were presented as mean ± s.e.m. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, ns no significance.

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