Table 3 Key antibodies used in flow cytometry.

From: Adipocytes regulate monocyte development through the OGT-NEFA-CD36/FABP4 pathway in high-fat diet-induced obesity

Antibodies

Channel

Source

Identifier

Anti-mouse lineage cocktail (B220, CD3e, CD11b, Gr1, Ter119)

FITC

Biolegend

133301

Anti-mouse Sca1

PE-Cyanine7

eBioscience

25-5981-82

Anti-mouse C-kit

PE

eBioscience

12-1171-81

Anti-mouse Flt-3

APC

eBioscience

17-1351-82

Anti-mouse CD34

APC-700

eBioscience

56-0341-82

Anti-mouse IL7Ra

APC

Biolegend

135011

Anti-mouse CD16/CD32

PE-Cyanine5.5

eBioscience

45-0161-80

Anti-mouse CD11b

PE-Cyanine7

Biolegend

101216

Anti-mouse Ly-6C

PE

eBioscience

12-5932-82

Anti-mouse Ly-6G

APC

Biolegend

127614

  1. FITC Fluorescein Isothiocyanate, PE Phycoerythrin, APC Allophycocyanin.